COL3A1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	ELISA. RIA. Western blot. Immunofluorescence: 1/40. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Incubation Time: 60 min at RT or 2-8°C over night (please see also "Protocols"). Positive Control: chicken skin or liver.  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Reactivity	Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Collagen type III from Chicken skin.
Specificity	Chicken Collagen type III: 100% Chicken Collagen type I: < 5 %. Cross-reaction with Human, Mouse, Rat, Bovine Collagen Type III: < 0,1 %.
Formulation	State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 0.1 ml distilled water, for further dilution use appropriate antibody diluent.
Concentration	lot specific
Purification	Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen, type III, alpha 1
Database Link	Entrez Gene 396340 Chicken UniProt ID P12105
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
Synonyms	COL3A1
Reference Data

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