ID3 Mouse Monoclonal Antibody [Clone ID: OTI8B3]

Specifications

Product Data
Clone Name	OTI8B3
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	12.8 kDa
Gene Name	inhibitor of DNA binding 3, HLH protein
Database Link	NP_002158 Entrez Gene 3399 Human UniProt ID Q02535
Background	Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA. [supplied by OMIM]
Synonyms	bHLHb25; HEIR-1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways	TGF-beta signaling pathway

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