PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI1B7]

Specifications

Product Data
Clone Name	OTI1B7
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	83.5 kDa
Gene Name	protein kinase C epsilon
Database Link	NP_005391 Entrez Gene 18754 MouseEntrez Gene 29340 RatEntrez Gene 609934 DogEntrez Gene 714533 MonkeyEntrez Gene 5581 Human UniProt ID Q02156
Background	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]
Synonyms	nPKC-epsilon; PKCE
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction

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