PKR (EIF2AK2) Mouse Monoclonal Antibody [Clone ID: OTI3F5]

Specifications

Product Data
Clone Name	OTI3F5
Applications	IHC, WB
Recommend Dilution	WB 1:500, IHC 1:500
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 51-191 of human EIF2AK2 (NP_001129123) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	61.9 kDa
Gene Name	eukaryotic translation initiation factor 2 alpha kinase 2
Database Link	NP_001129123 Entrez Gene 5610 Human UniProt ID P19525
Background	The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Synonyms	EIF2AK1; LEUDEN; PKR; PPP1R83; PRKR
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors

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