Basophils Mouse Monoclonal Antibody [Clone ID: 212H6.16]

Specifications

Product Data
Clone Name	212H6.16
Applications	FC, IHC, WB
Recommend Dilution	Cell sorting, ImmunoHistoChemistry frozen sections, Western Blot.  Applications tested: MACS- separation and flow cytometry, IHC frozen section.  Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Enriched-human tonsillar pDCs
Specificity	Human basophils and mast cells.  Species cross-reactivity: no-cross reactivity on Mouse Basophil.
Formulation	State: Purified State: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
Concentration	lot specific
Purification	QMA Hyper D Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles.
Background	Basophils and mast cells are multifunctional hematopoietic effector cells involved in allergic and inflammatory reactions. Immediate allergy is caused by release of chemical mediators by these cell types. In human peripheral blood, 212H6.16 is an antibody specific to a yet non-identified cell-surface structure present on basophils. 212H6.16 does not induce basophil degranulation, and is therefore a valuable tool for the isolation and study of human basophils. Tonsillar epithelium and skin keratinocytes are also stained by 212H6 in situ: antigen expression in tonsil is restricted to cells lining the epithelial crypts. (Bendriss-Vermare N.et al, Leukocyte Typing VII, White Cell Differentiation Antigen MC13, part 9, myeloid cells, 425-30, 2001; Editor Mason).
Synonyms	Basophil cells
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.