Cytokeratin 10 (KRT10) Mouse Monoclonal Antibody [Clone ID: DE-K10]

Specifications

Product Data
Clone Name	DE-K10
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunoblotting.  Flow Cytometry. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections.  Recommended Dilutions: 1/100–1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for Immunoblotting applications.
Reactivity	Canine, Feline, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal preparation extracted from Human epidermis.
Specificity	DE-K10 reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 10
Database Link	Entrez Gene 3858 Human UniProt ID P13645
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Synonyms	Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10
Reference Data

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