Serum Amyloid A (SAA1) Mouse Monoclonal Antibody [Clone ID: 115]

Specifications

Product Data
Clone Name	115
Applications	ELISA, WB
Recommend Dilution	ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human SAA detection in combination with a monoclonal tracer/detection antibody (HRP conjugated, Cat.-No DM1004HRP or Biotin conjugated Cat.-No DM1004B). Suggested Capture Coating Dose: 0.3 µg/well (Substrate: TMB). If the above suggested conditions are followed, approximately 1.1 ng/ml of SAA in serum/plasma or 0.6 ng/mL of SAA in medium can be detected with an assay range of 0 to 80 ng/ml. Western Blot: An antibody concentration of 0.1-0.5 µg will allow visualization of 100 ng/lane of recombinant Human SAA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified recombinant human SAA (MW: 12 kDa)
Specificity	This monoclonal antibody reacts with natural and recombinant Human SAA. Does not show any cross-reaction with other human cytokines or growth factors tested such as IL-1 beta, IL-8, MCAF, TGF-beta and EGF.
Formulation	0.01M PBS, pH 7.2 State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Double distllated water is recommended to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	serum amyloid A1
Database Link	Entrez Gene 6288 Human UniProt ID P0DJI8
Background	The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis.
Synonyms	SAA1, SAA2
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.