Midkine (MDK) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot. Immunohistochemistry.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human Midkine produced in E.Coli.
Specificity	This antibody recognizes Human Midkine.
Formulation	Azide Free, 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2 State: Aff - Purified State: Lyophilized purified IgG fraction Preservative: None
Reconstitution Method	Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Immunoaffinity chromatography on a column with immobilized recombinant Human Midkine
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	midkine (neurite growth-promoting factor 2)
Database Link	Entrez Gene 4192 Human UniProt ID P21741
Background	Midkine (MK, also called neurite growth promoting factor 2, NEGF-2), a product of a retinoic acid responsive gene, is a secreted 13 kDa protein belonging to the family of heparin binding growth/differentiation factors. MK shares 45% sequence identity with other member of this family called Pleiotrophin (HB-GAM). Midkine is composed of two domains held together by disulfide linkages. The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity. Part of the MK activity is enhanced by dimerization of MK. Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation. In the adult MK expression is restricted. In addition to normal development, MK is also involved in the pathogenesis of diseases e.g. inflammatory diseases, human carcinomas such as esophageal, stomach, colon, pancreatic, thyroid, lung, urinary, hepatocellular, neuroblastoma, glioblastoma, Wilm´s tumor etc. High MK levels are associated with poor prognosis in some type of cancer. The increased expresion in many carcinomas indicates that MK can be applied to the diagnosis of malignancy. Midkine is expressed during the reparative stage of bone fractures, also supresses infection of certain viruses including HIV in target cells. Anti-apoptotic and cell protecting activity of midkine makes it to be a promissing in therapy.
Synonyms	MK, MDK, MK1, NEGF2, Neurite outgrowth-promoting protein
Note	Quality Control Test Indirect ELISA - to determine titer of the antibody. SDS PAGE - to determine purity of the antibody.
Reference Data

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