FLT4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: 0.5-1.5 µg/ml, allows the detection of 0.25-0.5 ng/well rhVEGFR-3/FLT-4. Western Blot: 0.5-1 µg/ml, it will detect approximately 5 ng/lane of rh VEGFR-3/FLT-4 under reducing conditions depending on the visualisation method. Immunoprecipitation: 1-5 µg/ml lysate or reaction volume.  FACS: Use 1-5 μg/ml Immunofluorescence. Immunohistochemistry on Frozen sections.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant human soluble FLT-4 protein (110 kDa).
Specificity	The antibody will detect human VEGFR-3/FLT-4. In Western blots, this antibody shows a moderate cross-reactivity with VEGFR-2/KDR and a weak cross-reactivity with VEGFR-1/Flt-1.
Formulation	PBS, pH 7.4 containing no preservatives or stabilizers State: Azide Free State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1 mg/ml.
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	fms related tyrosine kinase 4
Database Link	Entrez Gene 2324 Human UniProt ID P35916
Background	The sVEGFR-3/FLT-4 monomers have a mass of approximately 120kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774). All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial
Synonyms	VEGFR3, FLT4, VEGF Receptor 3
Reference Data

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