PROX1 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunofluorescence: Use 1/750-1/1000 dilution and 1 µg/ml on cells. Western Bloting: Use 1-5 µg/ml. Immunohistochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human C-terminal part of the Homeobox Transcription Factor Prox-1 (homeo domain and prospero domain). (Cat.-No DA3565)
Specificity	This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by Immunostaining. This antibody cross reacts with Equine Prox-1 in Immunohistochemistry using Paraffin-Embedded Sections (See Figure).
Formulation	5mM PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with distilled sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Antigen-Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	prospero homeobox 1
Database Link	Entrez Gene 5629 Human UniProt ID Q92786
Background	Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.
Synonyms	PROX1
Reference Data

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