Vegfa Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: To detect Murine VEGF-A by direct ELISA a concentration of at least 0.5 µg/ml is required. This purified IgG, in combination with compatible secondary reagents, allows the detection of 0.5- 1.0 ng/well of recombinant Murine VEGF164 or other VEGF-A splice forms. Western blot: To detect Murine VEGF-A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections (Pretreatment: 0.01% Proteinase K, 15 min at RT).
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Murine VEGF164 Dimer (Cat.-No DA3518)
Specificity	The antibody recognizes Human and Murine VEGF-A under reducing and non-reducing conditions.
Formulation	PBS, pH 7.4 without preservatives and stabilizers State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	vascular endothelial growth factor A
Database Link	Entrez Gene 22339 Mouse UniProt ID Q00731
Background	VEGFA is a member of the PDGF/VEGF growth factor family and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternate transcriptional splice variants, encoding either freely secreted or cell associated isoforms, have been characterized. There is also evidence for the use of non AUG (CUG) translation initiation sites upstream of, and in frame with the first AUG, leading to additional isoforms.
Synonyms	VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Reference Data

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