IL10 Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Sandwich ELISA (assuming 100µl/well), a concentration of 8.0-10.0 µg/ml of this antibody will detect at least 1000 pg/well of recombinant Human IL-10 when used with Biotinylated Antigen Affinity purified anti-Human IL-10 (Cat.-No PP1032B) as the detection antibody at a concentration of approximately 0.05-0.1 µg/ml.  Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.  Immunohistochemistry on Paraffin Sections: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (>98%) E.coli derived Recombinant Human IL-10 (Cat.-No PA084).
Specificity	This antibody detects Human Interleukin 10. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified IgG fraction from Cell Culture
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Predicted Protein Size	~150 kDa
Gene Name	interleukin 10
Database Link	Entrez Gene 3586 Human UniProt ID P22301
Background	Interleukin 10 (IL-10) is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the interleukin-2 receptor) on macrophages and B lymphocytes. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R); a 560 amino acid, 110kDa glycoprotein whose expression can be induced in cultured macrophages and fibroblasts by elevated in HIV-1 infected individuals and has been implicated in the progression of the disease.
Synonyms	IL-10, CSIF, TGIF
Reference Data

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