Il1b Rabbit Polyclonal Antibody

CAT#: PP006B2

Il1b rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 50 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution ELISA:
To detect mIL-1 beta by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-1 beta.
Western Blot:
To detect mIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant mIL-1 beta.
Specificity Detect Murine IL-1 beta.
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration
of 0.1-1.0 mg/ml.
Purification Affinity chromatography.
Conjugation Biotin
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name interleukin 1 beta
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Synonyms IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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