Il6 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: b To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (3 pg/ml), a concentration of 0.005-0.008 µg/ml of this antibody is required. Direct ELISA: To detect mIL-6 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mIL-6. Sandwich ELISA: To detect mIL-6 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-0.2 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated antibody Cat.-No PP012B, allows the detection of 0.2-0.4 ng/well of recombinant mIL-6. Western Blot: To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant mIL-6
Specificity	This antibody recognises Interleukin-6 (IL-6).
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Murine IL-6 matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 6
Database Link	Entrez Gene 16193 Mouse UniProt ID P08505
Background	Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.
Synonyms	IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF
Note	Centrifuge vial prior to opening!
Reference Data

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