Ccl7 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Mouse MCP-3 by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 2000-4000 pg/well of recombinant Mouse MCP-3. Sandwich ELISA: To detect Mouse MCP-3 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Murine MCP-3 (Cat.-No PP015P) as a Capture antibody, allows the detection of at least 2000-4000 pg/well of recombinant mMCP-3.  Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Murine MCP-3 (Cat.-No P024)
Specificity	Reacts with Mouse Macrophage Chemotactic Protein-3 (MCP-3). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized, sterile filtred purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	chemokine (C-C motif) ligand 7
Database Link	Entrez Gene 20306 Mouse UniProt ID Q03366
Background	This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Synonyms	Small-inducible cytokine A7, CCL-7, MCP-3, C-C motif chemokine 7, SCYA6, SCYA7, NC28
Note	Centrifuge vial prior to opening!
Reference Data

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