Ccl7 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine MCP-3 (100.0 ng/ml), a concentration of 4.0–6.0 μg/ml of this antibody is required. Sandwich ELISA: To detect Mouse MCP-3 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MCP-3 (Cat.-No PP015B1 or PP015B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse MCP-3 Western Blot: To detect Mouse MCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse MCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Murine MCP-3.
Specificity	Reacts with Macrophage Chemotactic Protein-3 (MCP-3)
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing an immobilized Murine MCP-3 matrix
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month  or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	chemokine (C-C motif) ligand 7
Database Link	Entrez Gene 20306 Mouse UniProt ID Q03366
Background	This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Synonyms	Small-inducible cytokine A7, CCL-7, MCP-3, C-C motif chemokine 7, SCYA6, SCYA7, NC28
Note	Centrifuge vial prior to opening!
Reference Data

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