Ccl3 Rabbit Polyclonal Antibody

CAT#: PP017P2

Ccl3 rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, FN, WB
Recommend Dilution Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-1 alpha (10 ng/ml), a concentration of 2.0-5.0 µg/ml of a previous lot of this antibody was required.
ELISA:
(Indirect): To detect hMIP-1α by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-1α.
(Sandwich): To detect hMIP-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MIP-1α (PP017B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-1α.
Western Blot:
To detect mMIP-1α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant mMIP-1 alpha.
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity chromatography.
Conjugation Unconjugated
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name chemokine (C-C motif) ligand 3
Background Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.
Synonyms CCL-3, MIP-1-alpha, C-C motif chemokine 3, MIP1A, Small-inducible cytokine A3, SCYA3, LD78 alpha, G0S19-1 protein
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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