G CSF (CSF3) Rabbit Polyclonal Antibody

CAT#: PP1011P1

G CSF (CSF3) rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



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CNY 3,910.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Applications ELISA, FN, IHC, WB
Recommend Dilution Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human G-CSF (0.5 ng/ml), a concentration of 0.04-0.06 µg/ml of this antibody is required.
Sandwich ELISA: To detect Human G-CSF by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (Cat.-No PP1011B1 or PP1011B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human G-CSF.
Western Blot: To detect Human G-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: 0.25 µg/ml with an Overnight Incubation at 4°C.
This antibody stained Formalin-Fixed, Paraffin-Embedded Sections of Human angiosarcoma. An HRP-labeled polymer detection system was used with a DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly purified E.Coli derived recombinant Human G-CSF (Cat.-No PA060).
Specificity Recognizes Human Granulocyte Colony Stimulating Factor (G-CSF).
Other species not tested.
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing immobilized hG-CSF matrix.
Conjugation Unconjugated
Storage Condition

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Gene Name colony stimulating factor 3
Background Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.
Synonyms CSF3, GCSF, Pluripoietin, C17orf33
Note Centrifuge vial prior to opening.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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