GRO gamma (CXCL3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: Direct: To detect hGRO-gamma by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGRO-gamma. Sandwich: To detect hGRO-gamma by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human GRO-gamma (PP1014P1 or PP1014P2) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant hGRO-gamma. Western blot To detect hGRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-gamma is 1.5-3.0 ng/lane, under either recombinant hGRO-gamma.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hGRO-gamma (human GRO-gamma).
Specificity	Human Growth Related Oncoprotein gamma (GRO gamma).
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 3
Database Link	Entrez Gene 2921 Human UniProt ID P19876
Background	Growth Regulated Protein gamma (GRO gamma) is a cytokine that may play a role in inflammation and affects endothelial cells in an autocrine fashion. It belongs to the intercrine alpha family (small cytokine c-x-c) (chemokine cxc).
Synonyms	C-X-C motif chemokine 3, GRO-gamma, GRO3, GROG, SCYB3, MIP2-beta
Note	Centrifuge vial prior to opening!
Reference Data

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