GRO gamma (CXCL3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-gamma (100 ng/ml), a concentration of 1.0-2.0 µg/ml of this antibody is required. Indirect ELISA: To detect hGRO-gamma by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGRO-gamma. Sandwich ELISA: To detect hGRO-gamma by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GRO-gamma (Cat.-No PP1014B1 or PP1014B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGRO-gamma. Western Blot: To detect hGRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 0.7-0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Positive Control: Human Colon/Rectum Adenocarcinoma.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived  recombinant Human GRO-gamma (Cat.-No PA064)
Specificity	Human Growth Related Oncoprotein g (hGRO gamma)
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography using immobilized Human GRO-γ matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 3
Database Link	Entrez Gene 2921 Human UniProt ID P19876
Background	Growth Regulated Protein gamma (GRO gamma) is a cytokine that may play a role in inflammation and affects endothelial cells in an autocrine fashion. It belongs to the intercrine alpha family (small cytokine c-x-c) (chemokine cxc).
Synonyms	C-X-C motif chemokine 3, GRO-gamma, GRO3, GROG, SCYB3, MIP2-beta
Note	Centrifuge vial prior to opening!
Reference Data

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