KGF (FGF7) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Immunohistochemistry on Parafiin Sections: 0.5 µg/ml overnight at 4°C. This antibody stained formalin-fixed, paraffin-embedded sections of normal Human skin.  An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human KGF (25 ng/ml), a concentration of 0.56-0.85 µg/ml of this antibody is required. Direct ELISA: To detect Human KGF by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant Human KGF.  Sandwich ELISA: To detect Human KGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human KGF (Cat.-No PP1040B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hKGF.  Western blot: To detect Human KGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human KGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified (>98%) E.coli-derived 18.9 kDa recombinant Human FGF-7 (KGF)
Specificity	This antibody reacts with Human Keratinocyte Growth Factor (KGF). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Human KGF matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	fibroblast growth factor 7
Database Link	Entrez Gene 2252 Human UniProt ID P21781
Background	KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Synonyms	Keratinocyte growth factor, Fibroblast growth factor 7, HBGF7
Note	Centrifuge vial prior to opening!
Reference Data

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