Leptin (LEP) Rabbit Polyclonal Antibody

CAT#: PP1041P2

Leptin (LEP) rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hLeptin (7.5 ng/ml), a concentration of 0.5 - 3.0 µg/ml of this antibody is required.
ELISA: To detect hLeptin by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hLeptin.
Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: use at ~2 µg/ml for cryostat and paraffin sections.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant hLeptin
Specificity This antibody reacts with Human Leptin.
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity chromatography.
Conjugation Unconjugated
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name leptin
Background Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
Defects in the Leptin gene are the cause of profound obesity and type II diabetes.
Synonyms LEP, OB, OBS, Obesity factor, Obese protein
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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