Macrophage Inflammatory Protein 3 alpha (CCL20) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect MIP-3 alpha by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.15 - 0.3 µg/ml of this antibody is required. This antibody allows the detection of 0.2 ng/well of recombinant MIP-3 alpha.  Sandwich ELISA: To detect MIP-3 alpha by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This antibody allows the detection of 0.2 ng/well of recombinant MIP-3 alpha.  Western blot: This antibody can be used at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant MIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%), 8.0 kDa E.coli derived recombinant human MIP-3 alpha
Specificity	This antibody detects MIP-3 alpha.
Formulation	PBS, pH 7.2 Label: Biotin State: Sterile filtered, lyophilized Ig fraction
Reconstitution Method	Restore to a concentration of 0.1 - 1.0 mg/ml with sterile PBS containing 0.1% BSA.
Purification	Affinity chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 20
Database Link	Entrez Gene 6364 Human UniProt ID P78556
Background	Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. It binds to CCR6.
Synonyms	CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker
Reference Data

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