GM CSF (CSF2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Sandwich ELISA: To detect Human GM-CSF by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GM-CSF (Cat.-No PP1101B1 / PP1101B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect Human GM CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human GM CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 0.25 µg/ml with an overnight incubation at 4°C. HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGM CSF (1.0 ng/ml), a concentration of 0.13-0.20 μg/ml of this antibody is required.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human GM-CSF (Human Granulocyte Macrophage Colony Stimulating Factor)
Specificity	This antibody recognizes Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized purified (sterile filtered) Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized hGM-CSF matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	colony stimulating factor 2
Database Link	Entrez Gene 1437 Human UniProt ID P04141
Background	The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.
Synonyms	CSF2, GMCSF, Sargramostim, Molgramostin
Note	Centrifuge vial prior to opening!
Reference Data

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