Collagen II (COL2A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for Western blotting for highly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/200-1/1,000.
Reactivity	Bovine, Human, Mouse, Rat, Sheep
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen type II purified from adult Human knee and Bovine nasal cartilage. For immunization collagens extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein.
Specificity	Crossreactivity < 1% to other collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Formulation	0.1M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 Label: Biotin State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
Reconstitution Method	Restore with 0.1 ml deionized water (or equivalent)
Concentration	lot specific
Purification	Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	collagen type II alpha 1 chain
Database Link	Entrez Gene 1280 Human UniProt ID P02458
Background	Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Synonyms	COL2A1, Alpha-1 type II collagen
Reference Data

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