ADA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of adenosine deaminase [calf spleen] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature.  A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
Reactivity	Bovine, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Adenosine deaminase from calf spleen
Specificity	This antibody detects Adenosine deaminase [Calf spleen]. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Adenosine deaminase [Calf spleen].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig faction Stabilizer: 10 mg/ml BSA (immunoglobulin and Protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 280712 Bovine UniProt ID P56658
Background	Adenosine deaminase antibody is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. Present in virtually all mammalian cells, its primary function in humans is the development and maintenance of the immune system. However, the full physiological role of ADA is not yet completely understood.
Synonyms	Adenosine aminohydrolase, ADA
Reference Data

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