ADH1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/5,000-1/25,000. Western Blot: 1/500-1/2,000, expect a band approximately 37 kDa in size corresponding to monomeric alcohol dehdrogenase in the appropriate cell lysate or extract.
Reactivity	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Full length Alcohol Dehydrogenase isolated from yeast.
Specificity	Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Sodium Azide as preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00330
Background	Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.
Synonyms	Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947
Note	Protein Sequence: Yeast Alcohol Dehydrogenase, 347 aa, predicted MW 36.7 kDa 1 mtipdkqlaa vfhthggpen vkfeevpvae pgqdevlvni kytgvchtdl halqgdwplp 61 akmpligghe gagvvvkvga gvtrlkigdr vgvkwmnssc gnceycmkae eticphiqls 121 gytvdgtfqh ycianathat iipesvplev aapimcagit cyralkeskv gpgewicipg 181 aggglghlav qyakamamrv vaidtgddka elvksfgaev fldfkkeadm ieavkaatng 241 gahgtlvlst spksyeqaag farpgstmvt vsmpagaklg adifwltvkm lkicgshvgn 301 ridsiealey vsrglvkpyy kvqpfstlpd vyrlmhenki agrivldlsk
Reference Data

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