Alkaline phosphatase / ALPI / IAP Rabbit Polyclonal Antibody

CAT#: R1053

Alkaline phosphatase / ALPI / IAP rabbit polyclonal antibody, Serum



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CNY 20,681.00


货期*
4周

规格
    • 2 ml

Cited in 2 publications.

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Specifications

Product Data
Applications ELISA, IP, WB
Recommend Dilution Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of Alkaline Phosphatase [Calf intestine] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reactivity Bovine
Host Rabbit
Clonality Polyclonal
Immunogen Alkaline Phosphatase [Calf intestine].
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [Calf intestine].
Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
No reaction was observed against the bacterial form of the enzyme.
Formulation 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 without preservatives.
State: Serum
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore with 2.0 ml of deionized water (or equivalent).
Concentration lot specific
Purification Prepared from monospecific antiserum by a Delipidation and Defibrination.
Conjugation Unconjugated
Storage Condition Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Background Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Synonyms Intestinal alkaline phosphatase
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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