Beta-amylase Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/1000-1/5000. ELISA: 1/1,000-1/5,000 dilution suggested to begin optimization.
Reactivity	Sweet Potato
Host	Rabbit
Clonality	Polyclonal
Immunogen	Beta-amylase from sweet potato
Specificity	This antibody detects sweet potato beta-amylase. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-rabbit rerum as well as purified and partially purified beta- amylase [sweet potato].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P10537
Background	Beta-amylase is an enzyme found in the seeds of higher plants and in sweet potatoes. It hydrolyses 1,4 alpha D glucosidic linkages in polysaccharides to remove successive maltose units from the non reducing ends of the chains. Used in the structural analysis of starch and glycogen.
Synonyms	BMY1, AMYB
Reference Data

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