CPB1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/250-1/1,000 ELISA: 1/1,000-1/5,000 This product has been assayed against 1.0 µg of Carboxypeptidase B [porcine pancreas] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/3,000 of the reconstitution concentration is suggested.
Reactivity	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Carboxypeptidase B from porcine pancreas
Specificity	This product detects porcine Carboxypeptidase B. Cross reactivity against Carboxypeptidase B from other tissues and species is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Carboxypeptidase B [porcine pancreas].
Formulation	0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: HRP State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P09955
Background	Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates.
Synonyms	CPB1, CPB, PCPB, Pancreas-specific protein, PASP
Reference Data

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