DNASE1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Deoxyribonuclease I [Bovine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] code R1364HRP and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100 to 1:1,000 of the reconstitution concentration is suggested for this product.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Deoxyribonuclease I from bovine pancreas.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Deoxyribonuclease I [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease I from other tissues and species may occur but have not been specifically determined.
Formulation	0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 0.01% (w/v) Sodium Azide as preservative. State: Serum State: Lyophilized Serum
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Database Link	Entrez Gene 282217 Bovine UniProt ID P00639
Background	Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
Synonyms	DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I
Reference Data

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