DNASE1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000. Immunoprecitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Deoxyribonuclease 1 [bovine pancreas] in a capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2,500 of the reconstitution concentration is suggested.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Deoxyribonuclease 1 from bovine pancreas
Specificity	This antibody detects bovine Deoxyribonuclease 1. Cross reactivity against Deoxyribonuclease 1 from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresisgive a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Deoxyribonuclease 1 from bovine pancreas.
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 282217 Bovine UniProt ID P00639
Background	Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
Synonyms	DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I
Reference Data

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