GLUD1 Rabbit Polyclonal Antibody

CAT#: R1086

GLUD1 rabbit polyclonal antibody, Serum



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CNY 20,681.00


货期*
4周

规格
    • 2 ml

Cited in 1 publication.

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution This antibody has been tested for use in ELISA and by Western blot. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 62 kDa in size corresponding to glutamate dehydrogenase monomer subunit by Western blotting in the appropriate cell or tissue extract.
Recommended Dilutions:
ELISA: 1/4,000-1/16,000
Western blot: 1/1,000-1/3,000.
Reactivity Bovine
Host Rabbit
Clonality Polyclonal
Immunogen A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [Bovine Liver].
BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
State: Serum
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore with 2.0 ml of deionized water (or equivalent).
Concentration lot specific
Purification Delipidation and defibrination.
Conjugation Unconjugated
Storage Condition Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Gene Name glutamate dehydrogenase 1
Background Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.
Synonyms GLUD1, GLUD, GDH1
Note Protein sequence: Bovine GDH, 558 aa, predicted MW 61.5 kDa
1 myrylgeall lsragpaalg sasadsaall gwargqpaaa pqpglvppar rhyseaaadr
61 eddpnffkmv egffdrgasi vedklvedlk treteeqkrn rvrsilriik pcnhvlslsf
121 pirrddgswe viegyraqhs qhrtpckggi rystdvsvde vkalaslmty kcavvdvpfg
181 gakagvkinp knytdnelek itrrftmela kkgfigpgvd vpapdmstge remswiadty
241 astighydin ahacvtgkpi sqggihgris atgrgvfhgi enfineasym silgmtpgfg
301 dktfvvqgfg nvglhsmryl hrfgakciav gesdgsiwnp dgidpkeled fklqhgtilg
361 fpkakiyegs ilevdcdili paasekqlpk snaprvkaki iaegangptt peadkifler
421 nimvipdlyl naggvtvsyf ewlnnlnhvs ygrltfkyer dsnyhllmsv qeslerkfgk
481 hggtipivpt aefqdrisga sekdivhsgl aytmersarq imrtamkynl gldlrtaayv
541 naiekvfrvy neagvtft
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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