HXK1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/500-1/1,000. ELISA: 1/1,000-1/2,500. This product has been assayed against 1.0 µg of Hexokinase [Yeast] in a standard capture ELISA using peroxidase conjugated strepavidin and ABTS as a substrate for 30 minutes at room temperature.
Reactivity	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Hexokinase  from Yeast
Specificity	This antibody detects Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Hexokinase [Yeast].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID P04806
Synonyms	HXK1, HKA, Hexokinase PI, Hexokinase-A
Reference Data

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