LPO Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500 - 1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000 - 1/20,000. This product has been assayed against 1.0 µg of Lactoperoxidase [bovine milk] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested.
Reactivity	Bovine
Host	Sheep
Clonality	Polyclonal
Immunogen	Lactoperoxidase from bovine milk
Specificity	This product detects bovine Lactoperoxidase. Cross reactivity against Lactoperoxidase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified Lactoperoxidase [bovine milk].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 280844 Bovine UniProt ID P80025
Synonyms	LPO, SAPX, Lactoperoxidase, EC=1.11.1.7, Salivary peroxidase, SPO
Reference Data

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