PKM (pan m-PK) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/500-1/2,000. ELISA: 1/4,000-1/20,000. This product has been assayed against 1.0 µg of Pyruvate Kinase [rabbit muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested for this product.
Reactivity	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Pyruvate Kinase from rabbit muscle
Specificity	This antibody detects rabbit Pyruvate Kinase. Cross reactivity may occur against other mammalian Pyruvate Kinase, but were not specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as  purified and partially purified Pyruvate Kinase [rabbit muscle].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 100008676 Rabbit UniProt ID P11974
Background	Pyruvate Kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Genetic defects of this enzyme cause the disease known as pyruvate kinase deficiency and can cause hemolytic anemia.
Synonyms	PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle
Reference Data

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