Meso_4146 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/1000. ELISA: 1/1000-1/2500; This product has been assayed against 1.0 µg of uricase [Bacillus species] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-goat IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature.  Immunoprecipitation: 1/100. Conjugation and most immunological methods require high titer and specificity.
Reactivity	Bacillus sp.
Host	Goat
Clonality	Polyclonal
Immunogen	Uricase from bacillus species
Specificity	This product detects uricase (Bacillus species). Cross reactivity against uricase from other sources is unknown. Immunoelectrophoresis gives a single precipitin arc against anti-goat serum as well as purified and partially purified uricase [Bacillus species].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 State: Purified State: Lyophilized purified Ig fraction Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q5WBJ3
Background	Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation.
Synonyms	URIC, URO, UOX, EC 1.7.3.3, Urate oxidase
Reference Data

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