Glucoamylase 1 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/500-1/3,000. ELISA: 1/5,000-1/25,000. This product has been assayed against 1.0 µg of Glucoamylase [Rhizopus] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/15,000 to 1/75,000 of the reconstitution concentration is suggested.
Reactivity	Rhizopus
Host	Goat
Clonality	Polyclonal
Immunogen	Glucoamylase from Rhizopus
Specificity	This antibody detects Rhizopus Glucoamylase. Cross reactivity against Glucoamylase from other sources is unknown.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Glucoamylase from Rhizopus.
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P07683
Background	Glucoamylase is an important industrial enzyme used in saccharification steps in both starch enzymatic conversion and in alcohol production. The catalytic domain degrades oligosaccharides from the non reducing end, releasing glucose, and the starch domain binds the enzyme to raw starch and to the cell wall.
Synonyms	Gluc 1
Reference Data

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