pgmB Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/500-1/3000. ELISA: 1/1,000-1/5,000 dilution suggested to begin optimization. This product has been assayed against 1.0 µg of beta-phosphoglucomutase [Lactococcus lacti] in a standard Sandwich ELISA using peroxidase conjugated affinity purified anti-goat IgG [H&L] (Rabbit) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested.
Reactivity	Lactococcus lacti
Host	Goat
Clonality	Polyclonal
Immunogen	Beta-phosphoglucomutase  (PGMB) from Lactococcus lacti
Specificity	This antibody detects beta-phosphoglucomutase from Lactococcus lacti. Immunoelectrophoresis gives a single precipitin arc against anti-Goat serum as well as purified and partially purified beta-phosphoglucomutase [Lactococcus lacti].  Cross reactivity against b-Phosphoglucomutase from other sources is unknown.
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 State: Purified State: Lyophilized purified Ig fraction Stabilizer: None Preservative: 0.01% Sodium Azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multistep process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term.  Avoid repeated freezing and thawing.
Database Link	UniProt ID P71447
Synonyms	Beta-phosphoglucomutase, Beta-PGM, Phosphoglucomutase beta, Glucose Phosphomutase
Note	Conjugates available. Please ask for details.
Reference Data

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