RUB1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western Blot: 1/500-1/2,000) and ELISA (1/1,000-1/5,000). Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. A 6 kDa band corresponding to yeast Rub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Reactivity	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	This purified antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Specificity	This purified polyclonal antibody reacts with yeast Rub1. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q03919
Background	Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, Hub1. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog Nedd8 are activated by the E1 ubiquitin-activating enzyme UBA2, that forms isopeptide linkages between thio esters. Nedd8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1-/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.
Synonyms	YDR139C
Reference Data

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