AVD Rabbit Polyclonal Antibody

CAT#: R1201B

AVD rabbit polyclonal antibody, Biotin



Need it in bulk or conjugated?
Get a free quote

CNY 40,481.00


货期*
4周

规格
    • 10 mg

Product images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Avidin [Hen Egg White] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:8,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Reactivity Chicken
Host Rabbit
Clonality Polyclonal
Immunogen Avidin [Hen Egg White]
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative
Label: Biotin
State: Lyophilized purified IgG fraction
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration lot specific
Purification A multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below
Conjugation Biotin
Storage Condition Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Gene Name avidin
Background The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Synonyms AVD
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...