Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody

CAT#: R1550HRPS

Alanine aminotransferase 1 / ALT1 sheep polyclonal antibody, HRP, Purified



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CNY 7,688.00


货期*
4周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Western blot: 1/1,000-1/5,000.
ELISA: 1/10,000-1/40,000.
This antibody has been assayed against 1.0 µg of Alanine transaminase [pig heart] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested.
Reactivity Porcine
Host Sheep
Clonality Polyclonal
Immunogen Alanine transaminase (ALT) from pig heart
Specificity This antibody detects porcine alanine transaminase. Cross reactivity against alanine transaminase from other sources is unknown.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/m BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.  Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Background Alanine transaminase (ALT) is also referred to as Glutamic pyruvic transaminase (GPT).
Synonyms GPT, AAT1, GPT1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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