Cd3e Rat Monoclonal Antibody [Clone ID: KT3]

CAT#: SM003PT

Cd3e rat monoclonal antibody, clone KT3, Purified



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CNY 2,570.00


货期*
4周

规格
    • 25 ug

Product images

Specifications

Product Data
Clone Name KT3
Applications FC, IHC
Recommend Dilution Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 1x10e6 cells. For optimal staining incubations should be performed at room temperature.
Immunohistochemistry on Frozen Sections.
For optimal staining incubations should be performed at room temperature.
Reactivity Mouse
Host Rat
Clonality Monoclonal
Immunogen Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
Specificity This antibody recognizes the Mouse CD3 antigen, which is expressed by mature T cells.
The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
Formulation PBS, pH7.4 containing 0.09% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Affinity Chromatography on Protein G.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name CD3 antigen, epsilon polypeptide
Background Cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms T3/Leu-4
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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