CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: AT10]

Specifications

Product Data
Clone Name	AT10
Applications	ELISA, FC, FN, IHC, IP
Recommend Dilution	ELISA.  Functional assays.  Immunoprecipitation: 20 µg/ml. Flow Cytometry: Use 10 µl of 20 µg/ml diluted CD32 antibody to label 1x106 cells or 100 µl whole blood. Immunohistochemistry on Frozen Sections: 1/500-1/1000.  The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. It is recommended to use acetone fixation for frozen sections. Histology Positive Control tissue: Lymph node. Not suitable for Paraffin Sections.
Reactivity	Canine, Human, Monkey, Rhesus Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	K562 cell line
Specificity	This Monoclonal antibody AT10 recognises the Human CD32 antigen.  Clone AT10 blocks the binding of IgG to Fc gamma RII (Ref. 4).
Formulation	PBS without preservatives State: Low Endotoxin State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 20°C only. Avoid repeated freezing and thawing.
Gene Name	Fc fragment of IgG receptor IIa
Database Link	Entrez Gene 2212 Human UniProt ID P12318
Background	CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
Synonyms	Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2
Note	Endotoxin Level: < 0.01EU/µg
Reference Data

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