Tumor necrosis factor (TNF-alpha) Rat Monoclonal Antibody [Clone ID: MP6-XT3]

Specifications

Product Data
Clone Name	MP6-XT3
Applications	ELISA, FC, WB
Recommend Dilution	Western blot. ELISA: 5 µg/ml. Flow Cytometry: Membrane permeabilisation is required for this application.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Recombinant murine TNF-alpha expressed in E. coli.
Specificity	This antibody is specific for tumour necrosis factor alpha (TNF alpha), also known as TNFSF2. Clone MP6-XT3 is reported to neutralise the effects of mouse TNF alpha. We recommend the use of SM112A for this purpose.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 21926 Mouse UniProt ID P06804
Background	This 17kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immunoregulatory role.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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