Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: DC-10]

CAT#: SM1360PS

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone DC-10, Purified



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CNY 3,140.00


货期*
5周

规格
    • 100 ug

Cited in 3 publications.

Product images

Specifications

Product Data
Clone Name DC-10
Applications ELISA, IF, IHC, IP, WB
Recommend Dilution ELISA: This antibody clone DC-10 has been tested as the detection antibody in a Sandwich ELISA for analysis of Cytokeratin 18 in combination with the clone C-04 (Cat.-No SM3043P).
Positive Control: MCF-7 Human breast adenocarcinoma cell line. 
Flow Cytometry: 1-4 µg/ml. Intracellular staining.
Immunoprecipitation.
Western Blotting(Non-Reducing Conditions): 1-2 µg/ml, overnight in 4°C.
Positive Control: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. 
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
SDS-PAGE (10% separating gel). 
Immunocytochemistry.
Positive ControlMCF-7 Human breast adenocarcinoma cell line, HeLa Human cervix carcinoma cell line.
Immunohistochemistry on Paraffin Sections: 10 µg/ml. 
Positive tissue: Epithelial tissue (skin), colon.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Human breast carcinoma cell line PMC-42
Specificity The antibody reacts with Human Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. 
Negative Species: Porcine, Mouse, Rat, Hamster, Bovine, Canine (Dog), Sheep.
Formulation PBS, pH~7.4
State: Purified
State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Preservative: 15 mM Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage Condition

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Gene Name keratin 18
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Synonyms Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

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