PARP1 Mouse Monoclonal Antibody [Clone ID: A6.4.12]

CAT#: SM1399P

PARP1 mouse monoclonal antibody, clone A6.4.12, Aff - Purified



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CNY 4,587.00


货期*
4周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name A6.4.12
Applications ELISA, IHC, IP, WB
Recommend Dilution ELISA.
Immunoprecipitation.
Western Blot: 1/1000-1/5000.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose.
Reactivity Drosophila, Hamster, Human, Mouse, Rat, Xenopus
Host Mouse
Clonality Monoclonal
Immunogen Human PARP. Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.
Specificity This antibody recognises poly (ADP-ribose) polymerase (PARP).
Formulation State: Aff - Purified
State: Liquid purified IgG containing 0.09% Sodium Azide as preservative.
Concentration lot specific
Purification Affinity Chromatography on Protein G.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name poly(ADP-ribose) polymerase 1
Background PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.
Synonyms PARP-1, ADPRT, ADPRT 1, PPOL
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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