Il6 Rat Monoclonal Antibody [Clone ID: MP5-32C11]

Specifications

Product Data
Clone Name	MP5-32C11
Applications	ELISA, FN, IP, WB
Recommend Dilution	Functional assays. Immunoassays. Immunoprecipitation. Western blot: The typical starting working dilution is 1:10. The antibody can be used for immunochemical investigations of mouse IL-6 in structural studies and for quantitative/qualitative immunochemical detection methods. The antibody neutralizes the biological activity of mouse IL-6. Furthermore the antibody is useful for Western blot and as tracer antibody use in immuno assay procedures.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	The antibody neutralizes mouse interleukin 6 (IL-6). It does not cross-react with LPS (the component of the bacterial cell wall which is considered responsible for the toxicity of gram-negative bacteria) and TNF-alpha nor does it block human or rat IL-6 in proliferation assays using the KD83 cell line. MP5-32C11 inhibits mouse IL-6-induced proliferation of the NFS60 myelomonocytic cell line and KD83 plasmacytoma.
Formulation	PBS State: Azide Free State: Lyophilized Ig fraction
Reconstitution Method	Reconstitute the vial by injection of 0.5 ml sterile distilled or de-ionised water (Caution: vial is under vacuum).
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	interleukin 6
Database Link	Entrez Gene 16193 Mouse UniProt ID P08505
Background	IL-6 is a pluripotent 20-22 kDa cytokine which plays a role in the pathophysiology of severe infection and regulates the immune response, acute phase reaction and haematopoiesis. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. Continuous IL-6 gene expression makes an essential contribution to a multistep oncogenesis of plasma cell neoplasia. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas.
Synonyms	IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF
Reference Data

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