Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: 12G10]

CAT#: SM1740PT

Integrin beta 1 (ITGB1) mouse monoclonal antibody, clone 12G10, Purified



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CNY 2,570.00


货期*
4周

规格
    • 25 ug

Product images

Specifications

Product Data
Clone Name 12G10
Applications ELISA, FC, WB
Recommend Dilution Flow Cytometry: 1/25-1/50
ELISA: 10 µg/ml.
Western Blot.
Note: Clone 12G10 has been reported for use in Immunoprecipitation. However, due to the nature of the epitope recognised by clone 12G10, we do not recommend this application.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.
Spleen cells from an immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
Specificity This antibody recognises the beta1 integrin. This antibody has been shown to bind to a ligand induced binding site antibody, binding being increased in the presence of Fibronectin ligand.
This antibody also enhances alpha 5-beta 1 - Fibronectin interactions.
Does not react with Rat and Mouse.
Formulation PBS containing 0.09% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Affinity Chromatography on Protein G.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name integrin subunit beta 1
Background Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Synonyms Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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