CD146 (MCAM) Mouse Monoclonal Antibody [Clone ID: OJ79c]

Specifications

Product Data
Clone Name	OJ79c
Applications	ELISA, FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted CD146 antibody to label 106 cells in 100 µl. Clone OJ79c (Purified antibody Cat.-No. SM1860P/PT) has been successfully used in ELISA and Immunohistochemistry on Frozen Sections.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
Specificity	This antibody recognises the 118 kD cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells.
Formulation	PBS Label: FITC State: Liquid purified IgG fraction. Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	melanoma cell adhesion molecule
Database Link	Entrez Gene 4162 Human UniProt ID P43121
Background	CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to disease progression.
Synonyms	MCAM, MUC18, CD146
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.